A novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis.
|Title||A novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Coll O, Villalba A, Bussotti G, Notredame C, Gebauer F|
Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation that requires two sequences in the 3' untranslated region (UTR) of vertebrate substrates: the polyadenylation hexanucleotide, and the cytoplasmic polyadenylation element (CPE). Using a cell-free Drosophila system, we show that these signals are not relevant for Toll polyadenylation but, instead, a "polyadenylation region" (PR) is necessary. Competition experiments indicate that PR-mediated polyadenylation is required for viability and is mechanistically distinct from the CPE/hexanucleotide-mediated process. These data indicate that Toll mRNA is polyadenylated by a noncanonical mechanism, and suggest that a novel machinery functions for cytoplasmic polyadenylation during Drosophila embryogenesis.