A novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis.

TitleA novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis.
Publication TypeJournal Article
Year of Publication2010
AuthorsColl O, Villalba A, Bussotti G, Notredame C, Gebauer F
JournalGenes Dev.
Volume24
Pagination129–134
Date PublishedJan
Abstract

Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation that requires two sequences in the 3' untranslated region (UTR) of vertebrate substrates: the polyadenylation hexanucleotide, and the cytoplasmic polyadenylation element (CPE). Using a cell-free Drosophila system, we show that these signals are not relevant for Toll polyadenylation but, instead, a "polyadenylation region" (PR) is necessary. Competition experiments indicate that PR-mediated polyadenylation is required for viability and is mechanistically distinct from the CPE/hexanucleotide-mediated process. These data indicate that Toll mRNA is polyadenylated by a noncanonical mechanism, and suggest that a novel machinery functions for cytoplasmic polyadenylation during Drosophila embryogenesis.

URLhttp://dx.doi.org/10.1101/gad.568610
DOI10.1101/gad.568610