BlastR–fast and accurate database searches for non-coding RNAs.

TitleBlastR–fast and accurate database searches for non-coding RNAs.
Publication TypeJournal Article
Year of Publication2011
AuthorsBussotti G, Raineri E, Erb I, Zytnicki M, Wilm A, Beaudoing E, Bucher P, Notredame C
JournalNucleic Acids Res.
Volume39
Pagination6886–6895
Date PublishedSep
Abstract

We present and validate BlastR, a method for efficiently and accurately searching non-coding RNAs. Our approach relies on the comparison of di-nucleotides using BlosumR, a new log-odd substitution matrix. In order to use BlosumR for comparison, we recoded RNA sequences into protein-like sequences. We then showed that BlosumR can be used along with the BlastP algorithm in order to search non-coding RNA sequences. Using Rfam as a gold standard, we benchmarked this approach and show BlastR to be more sensitive than BlastN. We also show that BlastR is both faster and more sensitive than BlastP used with a single nucleotide log-odd substitution matrix. BlastR, when used in combination with WU-BlastP, is about 5% more accurate than WU-BlastN and about 50 times slower. The approach shown here is equally effective when combined with the NCBI-Blast package. The software is an open source freeware available from www.tcoffee.org/blastr.html.

URLhttp://dx.doi.org/10.1093/nar/gkr335
DOI10.1093/nar/gkr335