DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs
|Title||DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigó R, Johnson R|
CRISPR genome-editing technology makes it possible to quickly and cheaply delete non-protein-coding regulatory elements. We present a vector system adapted for this purpose called DECKO (Double Excision CRISPR Knockout), which applies a simple two-step cloning to generate lentiviral vectors expressing two guide RNAs (gRNAs) simultaneously. The key feature of DECKO is its use of a single 165?bp starting oligonucleotide carrying the variable sequences of both gRNAs, making it fully scalable from single-locus studies to complex library cloning.