Position-dependent electrostatic protection against protein aggregation.

TitlePosition-dependent electrostatic protection against protein aggregation.
Publication TypeJournal Article
Year of Publication2009
AuthorsBuell AK, Tartaglia GG, Birkett NR, Waudby CA, Vendruscolo M, Salvatella X, Welland ME, Dobson CM, Knowles TP
JournalChembiochem
Volume10
Pagination1309–1312
Date PublishedMay
Abstract

Proteins with a high propensity to aggregate can be largely prevented from doing so with surprisingly small changes to their primary structure. By using a combination of rational design and quantitative measurements of aggregation rates, we show that adding a single charge in specific "gatekeeper" regions is sufficient to change the timescale for amyloid fibril growth from minutes to weeks, thereby dramatically reducing the efficiency of this process.

URLhttp://dx.doi.org/10.1002/cbic.200900144
DOI10.1002/cbic.200900144